Journal: JCI Insight

Article Title: Single-cell profiling identifies impaired adaptive NK cells expanded after HCMV reactivation in haploidentical HSCT

doi: 10.1172/jci.insight.146973

Figure Lengend Snippet: ( A ) Gene sets involved in respiratory metabolism and enriched in KIR pos NK cells from NR and retrieved from GO. ( B ) Summary statistical graph showing the percentage of proliferating Ki67 pos /KIR pos NK cells (%, mean ± SD) in NR and R at 7–12 months after h-HSCT. ( C ) Summary statistical graph showing the percentage of KIR pos /NKG2C pos /IFN-γ pos NK cells in NR ( n = 3) and R ( n = 3) recipients at 8–12 months after h-HSCT either in the absence (-) or presence (+) of 721.221.G target cell lines. One-way ANOVA with Bonferroni correction. ( D ) Summary statistical graph showing the normalized value of PDC1 and LAG3 gene expression in FACS-sorted KIR pos NK cells from NR ( n = 8) and R ( n = 7). Paired t test. ( E ) Pearson correlation between PDCD1 and LAG3 gene expression on KIR pos NK cells. ( F ) Summary statistical graphs showing the percentage of IFN-γ pos NK cells in NR ( n = 8) and R ( n = 8) recipients at 8–12 months after h-HSCT, cocultured with HUVEC either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. ( G ) Summary statistical graphs showing the number of HUVEC cells infected with the BAC clone of the HCMV strain TB40/E expressing EGFP remained after 48 hours of coculture with KIR pos NK cells from NR ( n = 3) and R ( n = 3) either in the absence (-) or presence (+) of blocking antibodies (α–PD-1 and α–LAG-3). One-way ANOVA with Bonferroni correction. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The EndoGRO HUVECs (MilliporeSigma) were cultured in endothelial cell growth medium-2 (EGM-2) (Lonza) supplemented with 5% FBS, 1% Penicillin-Streptomycin, and 1% Ultra Glutamine in cell flasks precoated with Collagen I (Corning) at a density of 10 5 to 10 6 cells/mL in a humidified atmosphere at 37°C with 5% CO 2 .

Techniques: Gene Expression, Blocking Assay, Infection, Expressing

Journal: Cancers

Article Title: Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

doi: 10.3390/cancers12102850

Figure Lengend Snippet: Effects of PDIA1 inhibition on wound-healing and migration of breast cancer cells and endothelial cells. ECIS Wound-Healing Assay of hLMVEC, MCF-7 and MDA-MB-231 cells and breast cancer sublines with silencing of PDIA1 (shN, shPDIA1-1 and shPDIA1-3). Real time tracings in hLMVECs ( A ), MCF-7 ( C ) and MDA-MB-231 ( E ) cell lines after addition of bepristat 2a at various concentrations (1, 10, 30 or 50 µM) and after PDIA1 silencing in MCF-7 ( G ) and MDA-MB-231 ( I ) cell lines. Area under the curve boxplots represent AUC quantitation of changes in migration rate of bepristat 2a-treated hLMVECs ( B ), MCF-7 ( D ) and MDA-MB-231 ( F ) cell lines versus non-treated controls as well as MCF-7 ( H ) or MDA-MB-231 ( J ) sublines transduced against PDIA1 (shPDIA1-1, shPDIA1-3) or wild type cells regarding to negative sequence (shN). The line graphs and AUC boxplots represent mean ± SD of three independent experiments. Statistical analysis was calculated using parametric one-way ANOVA followed by Dunnett’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001).

Article Snippet: Human lung microvascular endothelial cell line (hLMVECs) was obtained from Cell Applications (San Diego, CA, USA).

Techniques: Inhibition, Migration, Wound Healing Assay, Quantitation Assay, Sequencing

Journal: Cancers

Article Title: Protein Disulphide Isomerase A1 Is Involved in the Regulation of Breast Cancer Cell Adhesion and Transmigration via Lung Microvascular Endothelial Cells

doi: 10.3390/cancers12102850

Figure Lengend Snippet: Effects of exogenous PDIA1 and PDIA3 on adhesive interaction between breast cancer cells and different substrates. Effect of exogenous proteins PDIA1 and PDIA3 on adhesion of MCF-7 ( A – F ) and MDA-MB-231 ( G – L ) cells to collagen type I, fibronectin and lung microvascular hLMVEC cells, respectively. Data represent mean ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test (* p = 0.05, ** p = 0.01, *** p = 0.001).

Article Snippet: Human lung microvascular endothelial cell line (hLMVECs) was obtained from Cell Applications (San Diego, CA, USA).

Techniques: Adhesive